|ELIMINATION OF MIXED WASTE GENERATION FROM NORTHERN BLOT WASHINGS|
Northern blotting is a method used in molecular biology to identify specific ribonucleic acid (RNA) fragments which have been resolved by gel electrophoresis via transfer of the band pattern to a filter matrix. A recent change to the Northern blot procedure has eliminated the generation of mixed waste (waste that contains chemicals regulated by the Resource Conservation and Recovery Act combined with radioactive materials) by substituting either methanol or ethanol with Sodium Chloride/Sodium Citrate (SSC) and Sodium Dodecylsulfate (SDS) during the blot washing.
10 CFR 20 Subpart K.
40 CFR Parts 260-268.
State of Michigan Act 451 Part 111.
Overview of Procedure
Set up Agarose gel with radiolabeled sample for electrophoresis. This will separate molecules by size for further work.
The membrane is now ready for further detection and analytical techniques.
& Health Precautions/Personal Protective Equipment
Another benefit to using the new procedure is the associated cost savings to the U-M. The cost of processing and disposal of aqueous LLRW is much less than that of mixed waste.
1. Liquid LLRW (non-mixed) $58 per gallon.
2. Liquid LLRW (mixed) $158 per gallon.
Therefore, the U-M's total saving is $100 for each gallon of mixed waste not generated by using the new blot washing procedure.