|REGENERATION OF DESTAINING SOLUTION FOR ELECTROPHORETIC GELS|
Once a protein of interest has been separated by gel electrophoresis, the gel can be stained using Coomassie Blue stain. Following the staining procedure, the gel is typically destained by soaking it in a 20 percent methanol/10 percent acetic acid solution.
If the protein has not been radiolabeled, it is possible to regenerate and reuse the destaining solution, thereby reducing the total volume of listed hazardous waste generated in the laboratory.
40 CFR Parts 260-268.
State of Michigan Act 451 Part 111.
Overview of Procedure
& Health Precautions/Personal Protective Equipment
Because the purchase price of laboratory reagents fluctuates, it is advisable to consult the U-M stores catalogue or your institution's purchasing department for current purchasing costs of methanol and acetic acid. Norit charcoal is available from the Sigma Chemical Company.
Disposal costs would be approximately $4.50 per liter for this type of waste, based upon current U-M costs. This represents a disposal cost savings of $22.50 ($4.50 per liter x 5 liters) for 50 cycles when the destaining solution is regenerated and reused.